Necrostatin-34

本公司产品仅用于科研实验，不得用于人体或动物的临床与诊断

Necrostatin-34 是一种 RIPK1 激酶抑制剂，通过占据激酶结构域中的独特结合袋，将 RIPK1 激酶稳定在非活性构象。

Necrostatin-34 is a RIPK1 kinase inhibitor, stabilizing RIPK1 kinase in an inactive conformation by occupying a distinct binding pocket in the kinase domain.(In Vitro):Necrostatin-34 (Nec-34) exhibits IC50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively.Necrostatin-34 (Nec-34, 10?μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation.Necrostatin-34 (Nec-34) may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase.

Necrostatin-34 (Nec-34) exhibits IC50 values of 667 nM and 134 nM for TNFα in FADD def-Jurkat cells and L939 cells, respectively.Necrostatin-34 (Nec-34, 10?μM) inhibits the dimerization-induced RIPK1 activation as examined by phosphorylation of Ser166 (p-S166) of RIPK1, a biomarker for RIPK1 activation.Necrostatin-34 (Nec-34) may block TNFα-induced complex II formation by inhibiting the activation of RIPK1 kinase. Western Blot Analysis Cell Line:RIPK1 knockout L929 cells transfected with an expressing vector encoding an inducible and dimerizable RIPK1 fused with FKBP at the C-terminus.Concentration:10?μM.Incubation Time:30 min (and then 100?ng/mL TNFα was added for indicated periods of time).Result:Downregulated p-S166 levels.

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2-{[3-cyano-4-(4-methylphenyl)-6-oxo-1,4

Apoptosis

RIP kinase

RIPK1

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375835-43-1

384.48

C18H16N4O2S2

>98% (HPLC)

DMSO:125 mg/mL (325.11 mM; Need ultrasonic)

O=C(NC1=NC=CS1)CSC2=C(C#N)C(C3=CC=C(C)C=C3)CC(N2)=O

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(-20℃)

With Ice Pack

≥ 2 years